嗜热泉生古细菌密码子使用偏向性的研究.docxVIP

嗜热泉生古细菌密码子使用偏向性的研究 在最重要的情况下，非正统的非正统的资源分配是不可能的。第一阶段的数据不是很可可执行的。这是一个确保治疗的异常迹象[15,16,17,18,19,20]。努力工作的发展报告了这一阶段的异常迹象[15、16、17、18、19、20]。 Aeropyrum pernix K1,as a subtype of Crenarchaeota microorganism,was isolated from a coastal solfataric(volcanic hydrothermal area that emits sulfuric gases)thermal vent at Kodakara-Jima Island,Japan in 1993.This organism is highly motile.Cells of A.pernix K1 are coccoid and are often surrounded by pili-like appendages.A.pernix K1 grows between70 and 100℃with optimal growth at 90-95℃.Although the genomic sequence of A.pernix K1 has been published,little genomic analysis is available on this microorganism.In this study,the synonymous codon usage of this microorganism is analyzed and a comparison is made with the other two phylogenetically related Crenarchaeota microorganisms(i.e.,Pyrobaculum aerophilum str.IM2 and Sulfolobus acidocaldarius DSM 639). 1 杏仁杏仁 1.1 invi结构 The complete genomes and the coding sequences of A.pernix K1 and two other Crenarchaeota microorganisms,including P.aerophilum str.IM2 and S.acidocaldarius DSM 639,were extracted from the NCBI Refseq project(Accession No.NC_000854,NC_003364,NC_007181,respectively). The sequence similarity is not enough to separate the orthologous genes from paralogous genes.So,only five genes with the similar names were selected from among the microorganisms,as orthologous genes,to investigate whether there was a correlation between the gene function and the codon usage bias.The five orthologous genes are shown in Table 1. 1.2 我 1.2.1 quequetrafteriecidiciding、gc3codons,trp To examine synonymous codon usage without the confounding influence of amino acid composition of different gene samples,the values of relative synonymous codon usage(RSCU)of different codons in each sequence were calculated.The RSCU value of the jth codon for the ith amino acid was calculated by where obsijis the observed number of the jth codon for the ith amino acid,which has nitype of synonymous codons.It is obvious