分子印迹固相萃取柱净化 HPLC-FLD 检测啤酒、葡萄酒及葡萄汁中赭曲霉毒素A.docVIP

  Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape  5 10 15 20 25 30 35 40 juice# CAO Jiliang1,3, KONG Weijun1, ZHOU Shujun1,4, YIN Lihui2, Wan Li3, YANG Meihua1** (1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193; 2. National Institutes for Food and Drug Control, Beijing 100050; 3. School of Pharmacy, Chengdu University of TCM, ChengDu 611137; 4. Jilin Agricultural University, ChangChun 130118) Abstract: A simple, reliable and low-cost method based on molecularly imprinted polymer as selective sorbent of solid-phase extraction was proposed for the determination of ochratoxin A in beer, red wine and grape juice by HPLC coupled with fluorescence detection (HPLC-FLD). Samples were diluted with water and cleaned up with an AFFINIMIP? SPE OTA column. After washing and eluting, the analyte was analyzed by HPLC-FLD. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) for ochratoxin A were 0.025 ng mL?1 and 0.08 ng mL-1, respectively. The recoveries of ochratoxin A from beer, red wine and grape spiked at 0.1, 2 and 5 ng mL-1 ranged from 91.6 to 101.7%. Furthermore, after a simple regenerated procedure, the MIP-based SPE column could be reused at least fourteen times to achieve more than 80% recoveries of ochratoxin A in real samples. The developed method was applied to the detection of thirty beer, red wine and grape juice samples and just only four samples were contaminated by ochratoxin A with levels below the legal limits. Keywords: Molecularly imprinted polymer; Solid-phase extraction; Ochratoxin A; Beer 0 Introduction Ochratoxin A is a widely distributed mycotoxin mainly produced by several fungal species of the genera Aspergillus and Penicillium [1]. OTA exhibits mu