HPLC determination of anti-asthmatic paste the content of amygdalin in.docVIP

 PAGE \* MERGEFORMAT 9 HPLC determination of anti-asthmatic paste the content of amygdalin in [Abstract] Objective To determine the anti-asthmatic plaster method of amygdalin content. Methods using high performance liquid chromatography using Inertsil ODS-3 C18 column (4.6 mm * 250 mm, 5 μ m), methanol -1 ‰ phosphoric acid (volume ratio seventy-nine past nine p.m.) as mobile phase; flow rate of 1.0 mL * min -1, detection wavelength was 210 nm. The results in the range of 0.12 ~ 1.2 μ g, the peak area and injection volume was a good linear relationship (r = 0.9998), recovery was 97.32%, RSD 1.07%. Conclusion The method is simple, reliable, accurate and satisfactory results. [Keywords:] HPLC; asthma paste; Amygdalin; Determination Determination of amygdaloside in Pingchuan patch by HPLC CHEN Qiu-fang, LI Hua, ZHOU Yi-sheng (Guangdong College of Pharmacy, Guangzhou, Guangdong 510006, China; Institute for Drug Control in Huangshi, Huangshi, Hubei 435000, China) Abstract: Objective To establish an HPLC method for determination of amygdaloside in Pingchuan patch.Methods The sample was analyzed on a Inertsil ODS-3 C18 column (4.6 mm * 250 mm, 5 μ m) using methanol-1 ‰ phosphate solution (21:79 ) as mobile phase. The flow rate was 1.0 mL * min-1 and the detection wavelength 210 nm. Results The method exhibited good linearity from 0.12 to 1.2 μ g (r = 0.9998). The average recovery was 97.32% and RSD 1.07%. Conclusion The method is convenient, reliable and accurate for the quality control of the Pingchuan patch. Keywords:: HPLC; Pingchuan patch; amygdaloside; content determination Asthma paste is ephedra, almonds and other traditional Chinese medicine made of topical preparations, with Xuanfei cold-dispelling asthma, heat Huatanzhike of efficacy for the treatment of cough asthma embolism. Amygdalin in bitter almonds, the main active ingredient [1,2]. In this paper, RP-HPLC determination of anti-asthmatic paste the content of amygdalin in results show that the