Applications of a new In vivo tumor spheroid based shell-less chorioallantoic membrane 3-D model in bioengineering research英文文献资料.docVIP

J. Biomedical Science and Engineering, 2010, 3, 20-26 JBiSE doi:10.4236/jbise.2010.31003 Published Online January 2010 (http://www.SciRP.org/journal/jbise/). Applications of a new In vivo tumor spheroid based shell-less chorioallantoic membrane 3-D model in bioengineering research Nzola De Magalh?es , Lih-Huei L. Liaw , Michael Berns , Vittorio Cristini , Zhongping Chen , 1 2 2 3 2 5 Dwayne Stupack 4 , John Lowengrub 1 2 3 4 5 Department of Biomedical Engineering, University of California, Irvine, USA; Beckman Laser Institute and Medical Clinic, University of California, Irvine, USA; Health Sciences Center, University of Texas, Houston, Houston, USA; Moores Cancer Center, University of California, San Diego, USA; Department of Mathematics, University of California, Irvine, USA. Email: nmmagalh@ Received 20 September 2009; revised 11 November 2009; accepted 3 December 2009. ABSTRACT closely reflect realistic solid tumors. While in vitro models may not provide a complete as- sessment of the processes that occur only in the envi- ronment of solid tumors [1], scientists have relied on tumor in vivo models or the integration of tumor in vitro models with in vivo models to circumvent the shortcom- ings in in vitro models. Current integration approaches typically introduce tumor cells to in vivo models as single cell suspensions, multi-layered systems such as biopsies, or embedded in scaffolds such as gels or sponges prior to implantation [2, 3]. Additionally, some investigators add exogenous fac- tors in the culture medium or scaffolds to induce certain functions such as angiogenesis [4] and invasion, that may manifest only in in vivo environments [5]. Single cell suspensions and biopsies may not be ideal tumor systems for bioengineering applic