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Branched RNA A New Architecture for RNA Interference英文文献资料.doc

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Branched RNA A New Architecture for RNA Interference英文文献资料

SAGE-HindawiAccesstoResearch JournalofNucleicAcids Volume2011,ArticleID586935,7pages doi:10.4061/2011/586935 ResearchArticle BranchedRNA:ANewArchitectureforRNAInterference AnnaAvi?no′, SandraM.Ocampo, Jos′eCarlosPerales, andRamonEritja 1 1 2 1 1 InstituteforResearchinBiomedicine(IRBBarcelona),InstituteofAdvancedChemistryofCatalonia(IQAC-CSIC), NetworkingCentreonBioengineering,Biomaterials,andNanomedicine(CIBER-BBN),BaldiriReixac10,08028Barcelona,Spain DepartmentofPhysiologicalSciences,SchoolofMedicine,UniversityofBarcelona(CampusBellvitge),FeixaLlarga, L’HospitaletdeLlobregat,08907Barcelona,Spain 2 CorrespondenceshouldbeaddressedtoRamonEritja,recgma@cid.csic.es Received16October2010;Accepted14January2011 AcademicEditor:ArthurvanAerschot Copyright?2011AnnaAvi?no′etal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Branched RNAs with two and four strands were synthesized. These structures were used to obtain branched siRNA. The branchedsiRNAduplexeshadsimilarinhibitorycapacityasthoseofunmodi?edsiRNAduplexes,asdeducedfromgenesilencing experimentsoftheTNF-αprotein.BranchedRNAsareconsiderednovelstructuresforsiRNAtechnology,andtheyprovidean innovativetoolforspeci?cgeneinhibition.AsthemethoddescribedhereiscompatiblewithmostRNAmodi?cationsdescribed todate,thesecompoundsmaybefurtherfunctionalizedtoobtainmorepotentsiRNAderivativesandcanbeattachedtosuitable deliverysystems. 1.Introduction In addition to these modi?cations, siRNA architecture isalsocrucialinthedesignofe?ectiveandspeci?csiRNA. Thearchitectureitselfcanbealteredbychemicalsynthesis. In addition to the canonical siRNA architecture of 21-nt Inrecentyears,siRNAshavegeneratedtremendousinterest in therapeutics [1]. Nevertheless, the transition of siRNAs from the laboratory to the clinical practice has encoun- tered several