茶渣分离茶多酚咖啡碱的研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.12 茶渣分离茶多酚咖啡碱的研究 陈明艳 （浙江迪耳化工有限公司，浙江金华 321016） 摘要：通过静态吸附试验考察茶渣对茶多酚的吸附性，然后考察了上样流速对茶渣分离茶多酚和咖啡碱的影响。结果表明，茶 渣能较好地去除茶多酚中的咖啡碱，但其茶多酚得率偏低；柱分离的最佳条件为：以2 mL/min的流速上样，水洗脱咖啡碱，80% 乙醇 洗脱茶多酚。洗脱液经浓缩，干燥，得到茶多酚产品，其中茶多酚含量在95%以上，EGCG含量大于60%，咖啡碱含量小于0.2%。 关键词：茶多酚；咖啡碱；茶渣；分离 文章篇号：1673-9078(2010)12-1358-1360 Separation of Tea Polyphenols and Caffeine by Tea Residue CHEN Ming-yan (Zhejiang Deyer Chemicals Co., Ltd, Jinhua 321016, China) Abstract: The static absorption of tea polyphenols (TP) by tea residue from tea extract was investigated in this paper, and the influences of the tea extract flow rate were also investigated. Results showed that caffeine could be removed effectively from tea extract by tea residue, but the yield of TP was much more less than D101 resin. The column separation condition was optimized and the best tea extract flow rate, caffeine eluent, and TP eluent were 2 mL/min, water and 80% aqueous ethanol, respectively. The final product was obtained by concentration and drying of TP eluent. The contents of tea polyphenols and EGCG in the final product were more than 95% and 60% respectively, while caffeine content was less than 0.2%. Key words: tea polyphenols; caffeine; macroporous adsorption resin; purification 茶多酚(Tea polyphenols, TP)又称茶鞣质或茶单 离的新材料。 宁，是茶叶中具有保健功能的重要物质，一般占茶叶 1 实验材料与仪器 干重的20~35%，其广泛应用于食品工业、农业、医药、 精细化工等领域，具有延缓衰老、抵抗病毒、美容护 1.1 实验材料 肤等作用，是一种常用的天然食品添加剂。从茶叶中 绿茶、D101吸附树脂、松木购自浙江金华市场， 提取茶多酚时，茶叶中的咖啡碱往往会一并被提取出 松木木质素实验室自制。 来，过多摄入咖啡碱会刺激中枢神经，增加血压，提