高效液相色谱法同时测定果汁中13种有机酸和白藜芦醇.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.12 高效液相色谱法同时测定果汁中 13种有机酸和白藜芦醇 陈意光，邓穗兴，柯振华，覃芳芳，叶嘉荣，邹树丹，郭克文 （广州市质量监督检测研究院，国家加工食品质量监督检验中心(广州)，广东广州 510110） 摘要：建立了果汁中 13 种有机酸和白藜芦醇的高效液相色谱测定方法。C18 色谱柱分离各种成分，水（pH ＝2.5 ）和甲醇作为 流动相，梯度洗脱。水果样品用榨汁机搅碎，高速离心，取上层果汁，加入等体积的水，混匀，过0.22 μm 滤膜后测试。果汁样品与 水等体积混合，过滤膜后测试。14 种化合物的方法检出限（S/N=3 ）在0.1 mg/L~8.9 mg/L 之间，线性相关系数均大于 0.996。鲜榨葡 萄汁、橙汁和苹果汁中 13 种有机酸和白藜芦醇的回收率大部分在 90%~110%之间，相对标准偏差均小于 5%。运用该方法测定了多 个鲜榨葡萄汁、橙汁、苹果汁和市售果汁及果汁饮料样品。 关键词：果汁；有机酸；白黎芦醇；高效液相色谱法 文章篇号：1673-9078(2010)12-1387-1390 Determination of 13 Kinds of Organic Acids and Resveratrol in Fruit Juice by HPLC CHEN Yi-guang, DENG Sui-xing, KE Zhen-hua, QIN Fang-fang, YE Jia-rong, ZOU Shu-dan, GUO Ke-wen (Guangzhou Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou 510110, China) Abstract: An HPLC method was developed for simultaneous determination of 13 kinds of organic acids and resveratrol in fruit juice. C18 reverse phase column and water (pH=2.5)-methanol dual solvent gradient elution system was used for fruit samples and the jam was centrifuged to get the clarified juice. The juice was then mixed with water with the ratio of 1:1. The limits of quantification (S/N=3) of the fourteen compounds were from 0.1 mg/L to 8.9 mg/L, while all the linear correlation coefficients were over 0.995 . Most of the recoveries of the compounds in grape juice, orange juice and apple juice were ranged from 90% to 110% with the relative standard deviation being of above 5%. This method can be used for the determination of contents of these compounds in fruit juice an