酶联免疫法 ELISA测定婴幼儿配方奶粉中的黄曲霉毒素M1方法改进.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.12 酶联免疫法（ELISA ）测定婴幼儿配方奶粉中的黄曲 霉毒素M1方法改进 梁迪思，李建生 （中国广州分析测试中心，广东广州 510070） 摘要：本文对ELISA检测婴幼儿配方奶粉中的黄曲霉毒素M 含量方法进行改进，处理方法为（1+1 ）甲醇水提取再经三氯甲烷净 1 化，提取效果比直接提取更佳。此方法的灵敏度为0.1 μg/kg，加标回收率为90.0%~105.6%，相对标准偏差为6.57%，方法重复性良好， 能有效消除假阳性。 关键词：酶联免疫法；婴幼儿配方奶粉；黄曲霉毒素M1 文章篇号：1673-9078(2010)12-1421-1423 Determination of Aflatoxin M in Infant Formula by Enzyme-linked 1 Immunosorbent Assay (ELISA) LIANG Di-si, LI Jian-sheng (China National Analytical Center, Guangzhou 510070, China) Abstract: In this study, aflatoxin M1 in infant formula was detected with ELISA kit, and its preparation was trichloromethane decontamination after extraction by the mixture of methanol and water (1:1, v/v). The results showed that the detection sensitivity was 0.1 μg/kg. The recoveries of aflatoxin M spiked at three levels were in the range of 88.9%~105.3%. This method had well reproducibility and its relative 1 standard deviation (RSD) in 6 parallel determinations was only 6.57%. This method was available to reduce false positive rate. Key words: ELISA; infant formula milk powder; aflatoxin M1 黄曲霉毒素M (Aflatoxin M , AFM )是动物摄入黄 法前处理步骤复杂，仪器设备要求高，样品需要免疫 1 1 1 曲霉毒素B (Aflatoxin B , AFB )后在体内经羟基化形 亲和柱作净化处理，价格昂贵。而酶联免疫吸附法是 1 1 1 成的代谢产物。AFM1主要存在于动物的乳、肾脏、肝 一种灵敏度、回收率较高的快速检测方法。但是，酶 脏、蛋、肉和尿中，其中以乳中最为常见[1] 。它是一种 联免疫试剂盒提供的方法一般只针对鲜乳样品，对于 强致癌物质，毒性仅次于AFB ，急性