同步荧光法结合主成分分析法鉴别橙汁品质.pdfVIP

162 2015,VoZ36,No．18艮晶科学 ※成分分析 同步荧光法结合主成分分析法鉴别橙汁品质 张晓丽，毛立新，范三红术 (山西大学生命科学学院，山西太原030006) 摘要：对鲜榨橙汁与市售某品牌橙汁进行同步荧光扫描得到其三维荧光光谱，并从光谱中提取出3组特征峰。然 后结合其中荧光物质的特性，得知这3组特征峰对应物质分别为VB：、VB。和黄酮类物质。同时，采用主成分分析 法结合于激发波长240～640am、发射波长与激发波长差为30am条件下扫描而得的同步荧光光谱数据对所有橙汁样 品进行聚类并识别，效果良好。结果表明：将荧光光谱技术与光谱模式识别方法相结合，可为橙汁品质鉴别提供有 力技术支持。 关键词：橙汁；同步荧光法；主成分分析 Fluorescence Combinedwith to Juice Synchronous Spectroscopy PrincipalComponentAnalysisIdentifyOrangeQuality ZHANG Xiaoli，MAOLixin，FANSanhong；I： ofLife Sciences，Shanxi 030006，China) (College University，Taiyuan fluorescencewasconductedon andcommercial Abstract：Synchronousscanning freshlypreparedorangejuice orange toobtainthe ofcharacteristicwereobtainedandbasedon three—dimensionalfluorescence peaks juice spectra．Threegroups theirfluorescence wereidentifiedas and thesame time， characteristics，thesepeaks VB2，VB6flavonoids，respectively．At the fluorescencedataofallthe obtained atexcitation synchronous spectral orangejuicesamples byscanning wavelengths of240--640nmwitha differenceof30nm totheemission were analyzedusingprincipal wavelength compared wavelength resultswereobtained thatthecombinationoffluorescence and componentanalysis(PCA)．Quitegood showing specgoscopy methodCan the technicalfortheidentificat