肌肉注射Ag85A+DNA疫苗对膀胱癌小鼠T细胞亚群和NK细胞活性的影响.pdfVIP

Materialsand methods Withthemethodofalkaline and lysis，A985A—V1Jns．tPA plasmid extractedand with i— V1Jns．tPAwere plasmid puffiedPolyethyleneGlycol，then was solatedona DNA toafinalconcentra— 0．8％agarosegel．Plasmidadjusted tionof insalineandstoredat一20℃． lg／L MBT一2 inoculated intothebackof cells(1×106cells)were intradermally 6—8weekoldC3H／HeNfemale tumorswereallowedto to5 mice，and develop to8mmindiameter。Thenmicewere dividedinto3 randomly groups：A985A— V1Jns．tPA group(experimentgroup)，V1Jns．tPAgroup(emptyplasmid control saline blank mousewas group)and group(normalgroup)．Each injec— tedwith or orsalineon femo— A985A——V1Jns．tpAVlJns．tpA quadriceps 100td risthree weeksafterthethird times(2一weekintervals)．2 vaccination，mice sacrificedand wereremoved ofCIM+T were spleens asepticly．The percentages andCD8+Tcellweremeasured flow thesame cell by performingcytometry．In OfNKcellswasdetected．Alldatawere asmean± time，cytotoxicity expressed t—testwasusedforstatistical SD，Student’s