奥沙利铂联合单次大剂量照射对大鼠直肠的损伤性分析-damage analysis of oxaliplatin combined with single high dose irradiation on rat rectum.docxVIP

Thestudyofratsrectumsdamageafterusingasinglelarge-doseradiatioandoxaliplatinAbstractObjective:ToinvestigatetheshorttermimpactofOxaliplatincombinedwithasinglelarge-doseofradiationonrectumdamageinrats,inordertoprovideareferenceforitsclinicalapplication.Methods:120ratswithoutanysignificantdifferenceinweightwereassignedrandomlyinto4groups:Agroup：chemotherapyalone（intra-peritonealinjectionofOxaliplatinat15mg/kg）；Bgroup:radiotherapyalone（6MVXray12Gy,singledoseradiationatratpelvic）；Cgroup：concurrentchemo-radiotherapy（intra-peritonealinjectionofOxaliplatinat15mg/kg,followedby6MVXray12Gysingledoseradiationatratpelvic).Dgroup：control（abdominalinjectionofequivalentamountofsaline）；with30rats/group.Theratsfromdifferentgroupsweresacrificedbyanesthesiaafterbeingirradiatedfor3,7,14,28,42days,with6rats/timepoint.MorphologicalchangeswereobservedbyHEstaining.Immunohistochemicalstreptavidin-peroxidase(S-P)methodwasusedtoquantifyBcl-2andBaxproteinexpressionintherectumtissue.Results:GroupCratshadthemostsevererectumgobletcellreduction,randomalignmentofwitheredglands,aswellaspresenceofalargequantityofinflammatorycellsandoccurrenceoffibrosis.ThesimilarreactionscouldalsobeseeningroupAandB，butthedegreewaslighter.ExpressionsofBaxweresignificantlydifferentbetweeneachexperimentalgroupandeachtimepoint（FBax＝25.151~40.396，P＜0.01）.TheexpressionofBaxineachexperimentalgroupwashigherthanthatinthegroupDonday3and7（F＝178.016~185.543，P＜0.01）,andthehighestwasthegroupConday7(p0.01).TheexpressionofBaxineachexperimentalgrouponday14,21,42waslowerthanthatinonday3and7,withstatisticalsignifican（cF＝174.152~195.069，P＜0.01）.ThepositiveexpressionrateofBcl-2significantlydifferentbetweeneachexperimentalgroupandeachtime(F＝10.644~66.889，p0.01).TheexpressionofBcl-2ineachexperimentalgrouponday7waslowerthanothereachpointinothertime（F＝418.076，P＜0.01）,andthelowestwasthegroupConday7(p0.01).Withtheextensionoftime,theexpressionrategraduallyuplifted.Withstatisticalsignificanchigherthanthecontrolgroup（F＝428.861~606.655，P＜0.001）,wit