咪喹莫特调节Th1Th2细胞相关趋化因子对兔耳增生性瘢痕创面愈合和瘢痕增生影响.docVIP

咪喹莫特调节Th1Th2细胞相关趋化因子对兔耳增生性瘢痕创面愈合和瘢痕增生影响 　　[摘要]目的：探讨咪喹莫特调节Th1、Th2细胞相关趋化因子对兔耳增生性瘢痕创面愈合时间和瘢痕增生的影响。方法：选取健康清洁级新西兰大耳实验兔40只，随机分为空白组、模型组、凡士林组以及咪喹莫特组，每组10只。模型组、凡士林组以及咪喹莫特组建立兔耳瘢痕模型，14d创面完全上皮化后，凡士林组涂抹凡士林软膏0.1g，1次/d；咪喹莫特组外用咪喹莫特软膏0.1g，1次/d，连续42d。观察并比较各组实验兔的大体情况，瘢痕增生指数，Th1、Th2 细胞相关趋化因子CXCL10、CXCL12、CCL2、CCL3、CCL5、CCL7、CCL13水平，完全上皮化时间及创面愈合时间。结果：模型组与凡士林组28d达到增生巅峰；与模型组相比，凡士林组及咪喹莫特组的瘢痕增生指数、创面愈合时间均?^低，且咪喹莫特组低于凡士林组，差异有统计学意义（P0.05）。结论：咪喹莫特能够调节Th1、Th2细胞相关趋化因子，缩短兔耳增生性瘢痕创面的愈合时间。 　　[关键词]咪喹莫特；趋化因子；兔耳瘢痕模型；创面愈合时间；增生性瘢痕 　　[中图分类号]R619+.6 [文献标志码]A [文章编号]1008-6455（2018）03-0100-04 　　Effect of Imiquimod Regulating Th1 and Th2 Cell-associated Chemokines on Wound Healing and Scar Proliferation in Rabbits 　　CHEN Fang 　　（Department of Medical Cosmetology，Linyi Central Hospital，Linyi 276400，Shandong，China） 　　Abstract： Objective To investigate the effect of imiquimod regulating Th1 and Th2 cell-associated chemokines in wound healing and scar proliferation in rabbits. Methods 40 healthy and clean New Zealand white rabbits were selected and randomly divided into the blank group， the model group， the vaseline group and the imiquimod group， 10 rabbits in each group. The model group， the vaseline group and the imiquimod group set up rabbit ear scar model. The vaseline group and the imiquimod group were given the appropriate treatment after 14 days of surgery， the vaseline group were treated with vaseline ointment 0.1g， 1 time/d， and the imiquimod group were treated with imiquimod ointment， treatment for 42 consecutive days. The level of scar hyperplasia index （SEI）， the levels of Th1 and Th2 cell-associated chemokines CXCL10， CXCL12， CCL2， CCL3， CCL5， CCL7 and CCL13， the complete epithelialization time and wound healing time were observed and compared. Results The model group and the vaseline group reached the peak of hyperplasia in 28 days. The scar proliferation index and wound healing time of the vaseline group and the imiquimod group were lower than those of the model group， and the imiquimod group