不同药物激活胆碱能抗炎通路对脓毒症大鼠心肌组织的保护作用-麻醉学专业论文.docxVIP

Abst Abstract IV IV ABSTRACT Objective: The cholinergic anti-inflammatory pathway is a neuroendocrine anti- inflammatory pathway, plays a key role in the regulation of inflammation. The experimental research of different drugs can protect the activation of cholinergic anti-inflammatory pathway in myocardial tissue. Methods: Establish clean male SD rat model of sepsis: Using the same rat 100 randomly divided into five experimental groups. Preserving skin after intraperitoneal injection of pentobarbital sodium anesthesia, By cecal ligation and puncture (CLP) to make the rat model of sepsis. Drug treatment after making model of sepsis: cholinesterase inhibitors neostigmine methyl sulfate， cholinergic agonist Kaba choline, highly selective alpha 2 adrenergic agonist dexmedetomidine hydrochloride in the same way take the immediate postoperative sepsis model by intravenous injection within 5 minutes after injection of the drug slow micro pump group，and open ligation appendectomy without drug-treated sham group and model of sepsis group without giving drug treatment.Intraperitoneal injection of Ringers solution 5ml / 100g rehydration after surgery，Excluding non-compliant after surgery. And record the quality of life and survival for each experimental group of rats. Brain natriuretic peptide (BNP) and high mobility group protein B1 (HMGB1) expression at 6 hours and 12 hours after the operation of making sepsis model ： half the rats were sacrificed 6 hours after each experimental group, immediately open blood. The remaining half of the rats in each experimental group were sacrificed 12 hours after surgery, immediately open blood. 4500 rpm for 8 minutes, extract serum, used enzyme-linked immunosorbent assay (ELISA) to measure expression of BNP and HMGB1. PAGE PAGE VI Observing the myocardial tissue HE staining pathological sections: 12 hours after surgery, the rest of the rats were sacrificed， Taking left ventricular tissue, with lower paraformaldehyde-fixed, p