成年期社会隔离对雄性BALBC小鼠神经行为学影响.docVIP

成年期社会隔离对雄性BALBC小鼠神经行为学影响 　　[摘要] 目的 探讨成年期社会隔离对雄性BALB/C小鼠神经行为学的影响。 方法 8周龄的雄性BALB/C小鼠随机分为群养组（GH组，n = 16）和社会隔离组（SI组，n = 16）。群养组作为对照，每笼饲养4只小鼠；社会隔离组单只单笼饲养。在40 d的社会隔离后进行一系列行为学测试，包括开放旷场实验（OFT）、新异物体辨别实验（NORT）、高架十字迷宫实验（EPMT）、强迫游泳测试（FST）、T迷宫（TMT）、社会互动测试（SIT）和入侵者诱发攻击实验（IEAT）。比较两组各测试结果。 结果 在FST中，社会隔离组的不动时间显著短于群养组，差异有统计学意义（P 0.05）；在EPMT中，社会隔离组在开臂停留的时间显著长于群养组，差异有统计学意义（P 0.05）；在TMT中，社会隔离组的正确率显著高于群养组，差异有统计学意义（P 0.05）；在SIT中，社会隔离组的社会交往行为的时间显著长于群养组，差异有统计学意义（P 0.05）。 结论 雄性BALB/C小鼠成年期社会隔离对其可能具有抗抑郁、抗焦虑、提高学习记忆能力和增加社会交往行为的作用。 　　[关键词] 社会隔离；成年期；神经行为学；BALB/C小鼠 　　[中图分类号] R338 [文献标识码] A [文章编号] 1673-7210（2016）06（c）-0013-05 　　[Abstract] Objective To investigate effects of social isolation on neuroethology in adult male BALB/C mice. Methods 8 weeks old male BLAB/C mice were randomly separated into housed group group （GH group， n = 16） and social-isolated group（SI group， n = 16）. GH group were as control， 4 mice in each cage， and mice of SI group were a mouse in single cage. A series of behavioral tests were performed after 40 d， including open field test （OFT）， novel object recognition test （NORT）， elevated plus maze test （EPMT）， forced swimming test （TST）， T-maze test （TMT）， social interaction test （SIT） and intruder-evoked aggressive test （IEAT）. The results of two groups were compared. Results In the FST， the immobility time of the SI group was significantly less than that of the GH group， the differences were statistically significant （P 0.05）. In the EPMT， the duration time in the open arms of SI group was significantly more than GH group， the differences were statistically significant （P 0.05）. In the TMT， SI group exhibited higher accuracy than GH group， the differences were statistically significant （P 0.05）. In the SIT， SI group displayed more social interaction than GH group， the differences were statistically significant （P 0.05）. Conclusion Social isolation in adulthood may have antidepressant， antianxiety， improving cognitive function and enhancing social interaction behav