重金属胁迫下微紫青霉菌菌株gxcr及突变体菌株sm1酶活性的研究-study on enzyme activity of penicillium microphyllum strain gxcr and mutant strain sm1 under heavy metal stress.docxVIP

THE RESEARCH ABOUT THE ACTIVITIES OF THE ENZYME IN Penicilliumjanthinellum strain GXCRAND UVMUTATNT (SMl) UNDER HEAVY MESTAL STRESS ABSTRACT We 串eparated Penicillium janthinellum strain GXCR that can re耐t to many heavy metal. In this study，we investigated growth and activíties oC some important enzymes(SOD 、PPO、POD、IAAO、CAT) under normal conditions and Trp，Cu今， Mn如 str剧崎量， discussed the cbanging of enzyme activiti创 under heavy metal stress. Using atom absrbing instrument to measure tbe ωntent of CU2+ in enchylema under different metal stress，80 we can find resisting mechanism of Penicillium jantbinellum GXCR. Growth quantity oC GXCR and its mutant(SMl)were lower under CU2+ treated than other sample嚣， there have bigber growth quantity under Trp+Cu飞Mnl+ treated. SOD oC GXCRl and its muta叫SM!) ascend under the kinds press，but other en勾Imes changing were different. It indiωted tbat irritating mechanisms of GXCR and its mutant(SMl) were different，maybe because of enzym础， harmony，or diCCerent genotype arose the differentia. At pl剧时， a few researches of mkroor百anisms irritating reaction under many a lot oC environment contamination presses，al50 researchωin this aspeωis lack in China. With the studying of microorganism irritatíng enzyme active under beavy metal，it can ofCer theoηgist oC microorganism irritating mechanism. Key: 日创vy metal Ac创vity of enzyme Peníci11iUIO 11 广回曹大摒喧JIf:l刊舍 广回曹大摒喧JIf:l刊舍 3比 第一意前言 1.1 重金属污染及生物修复 1. 1.1 重金属污绕 随着工业的发展，矿资源的开发利用，重金属污染的问题日益突出，重金属污染可分为大 气中的案余属污染、水体中的囊金属污染和土壤中的震金属污染[1. 2J 。大气中的震金属污染 主要哥来源于工业生产、汽车尾气排放及汽车轮胎磨损产生的大量含建金庸的有害气体和粉 尘等，它们主要分布夜工矿区的周围和公路、铁路的两侧 Iιη。工矿业废水、生活污水等米 经近 坦处lI P 向外排放，污染了土壤和水体，废弃物堆放场受流水作用以及窟含意金属的大 气沉降物输入，都使水体重金属含量急剧 11 高，导致水体受到重金属污染 [8-12J。金属矿床开发、 城市化、固体废弃物飨积以及为提高农业4:产而施用化肥、农药、污泥及污水灌溉过程中， 都可以便霆金属在土壤中大最积泉[1 3耐。目前，已有…些地方便产的粮食、疏菜、水果等 食物中烫金属含量超标或接近临界值，这些农产品通过食物链窗绕到人体和动物体中，还 会危及人类的生命和健康，引发撒症和其他疾病等，并导敖大气、地表水、地下水污染和 生态系统i段化等其他次致使态问题归]a 固体废弃物种类繁多，成分复杂，其危言事方式和污 染程度也不尽相同。其中，以矿业和