温泉瓶尔小草离体培养及种质试管保存培养基筛选.docVIP

温泉瓶尔小草离体培养及种质试管保存培养基筛选 　　摘 要：以温泉瓶尔小草新生幼叶为外植体，应用均匀设计法筛选其最适宜的离体培养和种质试管保存各阶段培养基.结果表明，最适合愈伤组织诱导的培养基为WPM+ZT0.50 mg#8226;L????-1??+2,4-D1.90 mg#8226;L????-1??，诱导率为??96.5%??；芽苗分化的培养基为WPM+ZT1.70 mg#8226;L????-1??+NAA0.10 mg#8226;L????-1??，分化率为99.0%；生根培养基为??1/4MS+IAA0.05 mg#8226;L????-1????+IBA0.03 mg#8226;L????-1??，生根率达97.8%以上；试管保存培养基为WPM+KT1.10 mg#8226;L????-1??+根皮苷3.20 mg#8226;L????-1??，通过24个月的保存，芽苗生长率仅在6.5%以下.试验结果证明，成功建立了温泉瓶尔小草离体培养体系，常温条件下利用延缓生长的方法在试管内保存温泉瓶尔小草种质是可行的.?? 　　关 键 词：温泉瓶尔小草；种质试管保存；均匀设计；根皮苷 　　中图分类号：S?@722 文献标志码：A 文章编号：1008-9497(2011)02-205-06 　　 　　Journal of Zhejiang ??University??(Science Edition), 2011,38(2):205-210 　　Abstract: The tender leaves of ??Ophioglossum thermale?? Kom. were used as explants, all suitable media of culture ??in vitro?? and germplasm conservation ??in vitro?? were screened through uniform design experiments. The results showed that WPM+ZT0.50 mg#8226;L????-1??+2,4-D1.90 mg#8226;L????-1?? was most suitable for callus induction with a induction rate of 96.5%; WPM+ZT1.70 mg#8226;L????-1?? +NAA0.10 mg#8226;L????-1?? was most suitable for shoots regeneration with a regeneration rate of 99.0%; 1/4MS+IAA0.05 mg#8226;L????-1?? +IBA0.03 mg#8226;L????-1?? was most suitable for rooting with a rooting rate of 97.8%; WPM+KT1.10 mg#8226;L????-1??+phloridzin3.20 mg#8226;L????-1??was most suitable for ??in vitro?? germplasm preservation for 24 months with a growing rate of 6.5%. The results prove that ??In vitro?? culture system of?? Ophioglossum thermale?? has been successfully established, and the method of defering growth was practical for germplasm conservation?? in vitro?? at normal temperature. 　　?お?Key Words: ??Ophioglossum thermale?? Kom.; germplasm conservation ??in vitro??; uniform design; phloridzin 　　?? 　　 　　温泉瓶尔小草(Ophioglossum thermale Kom.)又称狭叶瓶尔小草,是瓶尔小草科瓶尔小草属多年生草本植物，国家二级重点保护植物.《中国珍稀濒危保护植物名录（第一册）》中定为渐危种［1］，《吉林省野生动植物保护管理暂行条例》中定为省级一类重点保护植物.它是长白山区野生珍稀濒危药用植物，主治疔疮肿毒、目赤肿痛、乳痈、疔疮、疥疮身痒、跌打损伤、瘀血肿痛等.其株形优美，可引种用于湖畔、河岸、池边等湿地绿化的地被植物.其在长白山区数量十分