二苯并噻吩脱硫菌的筛选及其代谢途径研究-生态学专业论文.docxVIP

Abstract Dibenzothiophene (DBT) is a major heterocyclic organic sulfide in petroleum. It is very difficult to be degraded by traditional desulfurization method. Therefore, DBT is always considered as model compound for biodesulfurization research. In this article, a strain 6-1 which is capable of desulfurizing DBT as sulfur source was isolated from the waste water polluted by petroleum. The strain 6-1 was identified by analyzing 16S rDNA gene sequence. The searches were conducted with BLAST program in the GenBank (NCBI), which exhibited high levels of 99% similarity with Cronobacter turicensis strain. The growth curve of this strain indicated that the strain 6-1 stepped into logarithmic phase after 12 hours which lasted for about 50 hours. Then, the growth of strain 6-1 entered stationary phase which lasted for 40 hours. During stationary phase, cell concentration of the strain remained nearly invariable. The degrading curve of this strain indicated that DBT was degraded with the growth of the culture. From 36h to 48h, more than 80% DBT was degraded. Five single-factor experiments have been carried out to optimize the culture conditions for strain 6-1. Results showed the optimal culture conditions: 2g/L glycerol as carbon source; 2g/L ammonium chloride as nitrogen source; 0.2mM DBT as sulfur source; the most appropriate initial pH of culture media was 7.0 while the optimal temperature for growth was 30℃。 4The metabolites of DBT were identified by GC-MS analyses of dichloromethane extracts of the culture supernatants. The result indicates that the DBT ring is cleaved and 4 DBT is mineralized completely to CO2 , H2O and SO 2- by the culture. Key words：Biodesulfurization Dibenzothiophene Isolation Optimization Degradation pathway 独创性声明 本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得 的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他 个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集 体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。 学位论文作者签名： 日期： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有 权保留并向国家有关部门或机构送交论文的复印件和电子版，