姜黄素酯化物抗平滑肌细胞增殖和调血脂作用研究..docVIP

姜黄素酯化物抗平滑肌细胞增殖和调血脂作用研究 ：黄燕芬洪行球袁小凤 】［目的］研究姜黄素酯化物对不同条件促牛 主动脉平滑肌细胞增殖作用和对小鼠食饵性高脂血症的影 响，初步探讨其构效关系。［方法］用丁二酸酐酯化姜黄素 获得姜黄素酯化物；用oX-LDL或血管紧张素II培养牛VSMC 建立VSMC增殖模型，再在模型中加姜黄素和姜黄素酯化物 培养，用MTT法检测观察二者对平滑肌细胞增殖作用的影响; 用高脂膳食喂饲小鼠建立食饵性高脂血症模型，用姜黄素和 姜黄素酯化物进行实验性治疗，给药2周后处死小鼠，测定 血清总胆固醇、甘油三酯和高密度脂蛋白含量，计算低密度 脂蛋白含量，观察其调血脂作用。结果姜黄素酯化物浓度在 66 Pmol/L、33pmol/L、ymol/L 时，对 10mg/LoX-LD L 培 养的VSMC增殖有明显的抑制作用，P;剂量在/时，可降 低食饵性高脂血症小鼠血清TG含量P，但降TC、LDL C 和上调HDL作用不明显。［结论］姜黄素酯化物高剂量时能 明显抑制oX-LDL和Angll促牛VSMC增殖作用；能明显降低 血清TG降低食饵性高脂血症小鼠血清TG含量。 关键词】姜黄素酯化物平滑肌细胞调血脂 A bstract： [Objective ]Tostudytheeffects oftheanti prolifer ationoframificatio nsofthecurcuminest erifiedbysuccinica nhydrideontheBovin eVSMCandregulating thelipidsinhyperli pidemicmiceinduced byhighlipiddiettos tudytherelationbet weenstructureandef feet. [Methods] Cure uminwasesterifiedb ysuccinicanhydride ， MTTwasusedtoobser vetheanti prolifer ationoftheramifica tionsofthecurcumin esterifiedbysuccin icanhydrideonVSMCs timulatedbyox werefedwithhighlipiddietlweektobehyperli were fedwithhighlipiddi etlweektobehyperli pidemicmodel,andthengivenexperimenta pidemicmodel,andth engivenexperimenta ltreatmentwithcurcuminandramificatio ltreatmentwithcurc uminandramificatio nofcurcumin. Theleveloftotalcholester nofcurcumin. Thelev eloftotalcholester olandtriglycerideandHDLCinserumwere olandtriglyceridea ndHDL Cinserumwere allmeasuredinl51evelofLDL Cwascaculated. [Results] Theramificationofthecurcuminesterifiedhas Thera mificationofthecur cuminesterifiedhas obviouseffectontheinhibitionofVSMCst obviouseffectonthe inhibitionofVSMCst imulatedbylOmg/LoxLDLAndithaseffec imulatedbylOmg/Lox LDL Andithaseffec tsondecreasingthelevelofTGinserumoft tsondecreasingthel evelofTGinserumoft Conclu sion ]ifiedbysuccinicanhydridehasobviousef Conclu sion ] ifiedbysuccinicanh ydridehasobviousef hemiceinducedbyhig hlipiddiet. Theresultsind icatethatramificat ionofcurcuminester fectoninhibitionof VSMCstimulatedbylO mg/Lox LDLand2mg/L Ang II.Ithassignific anteffectsondecrea singTGinserum. Key