碱性染料间能量转移荧光分析的研究-分析化学专业论文.docxVIP

中文摘要中文摘要 中文摘要 中文摘要 本文共分五章。第一章为文献综述，对荧光共振能量转移技术的原理、分析 技术及其应用进行了较全面的综述。引用文献56篇。 第二章对碱性荧光染料吖啶橙(A0)、吖啶黄(AY)、罗丹明6G(R6G)、罗丹明 13(V,B)、乙基罗丹明B(ERB)、丁基罗丹明B(BRB)、碱性藏花红(ST)组成的各 种染料混合体系的能量转移进行了研究。研究了各种表面活性剂对碱性阳离子染 料间能量转移的影响，以及转移效率的影响因素。 第三章介绍了荧光能量转移在测定无机离子中的具体应用。第一节吖啶橙一 罗丹明6G能量转移荧光猝灭法测定痕量磷；第二节PAR一吖啶黄能量转移荧光 猝灭注测定痕量铁(II)的研究。 第四章罗丹明B～7一碘一8一羟基喹啉一5一磺胶束体系荧光熄灭法测定钒。 第血章苯基荧光酮荧光分光光度法测定粉煤狄中的痕量锗。 关键词 能量转移，荧光分析，染料，表面活性剂 AbstractAbstract Abstract Abstract This paper consists of five chapters．In the first paper,Application of fluorescence analysis based on resonance energy transfer(FRET)is reviewed with 56 references．The review includes F6rster theory of fluorescence resonarice energy transfer,analysis techniques and application． In the second chapter the fluorescence energy transfer between some basic dyes， such as acridine orange(AO)，acridine yellow(AY)，rhodamine 60(Rh60)，rhodamine B(Rhl3)，ethyl ester of rhodamine B(ERhB)，buthyrhodamine B(BRhB)and safranine T(ST)，was investigated．The effect of various surfactants Oil fluorescence energy transfer of basic dyes was studied，tOO．The conditions effecting energy transfer efficiency were discussed， Practical application of fluorescence energy transfer to the determination of inorganic ions was introduced in the third chapter．In the first section，the determination of trace phosphorus by using energy transfer fluorescence quenching from acridine orange to rhodamine 6G Was studied．In the second section，study on the energy transfer florescence quenching among combination of acridine yellow-PAR determination of trace iroll． In the fourth chapter，determination of trace Vanadium(v)by a fluorescence quenching method with rhodamine B一7一iodo一8·hydroxyquinoline。5一sulfonic acid surfactant system was studied． In the fifth chapter，spectrofluorometric determination of trace germanium with phenylfluorone in flyash was investigated． Keywords：energy transfer：f】uorescence analysis；dyes；surfactant； 第一章综 第一章综 述 =============================================== 第一章综 述 共