群体感应信号分子AI2高产乳酸菌株筛选及特性研究.docxVIP

群体感应信号分子AI-2高产乳酸菌株 筛选及特性研究 蔡针华程娜贾震虎逢晓阳 山西师范大学生命科学学院中国农业科学院农产品 加工研究所 摘要： 乳酸菌群体感应系统是乳酸菌响应环境变化、调节体内和关基因表达的重要调控 通路。为深入研究乳酸菌的群体感应系统，对8株乳酸菌产群体感应信号分子自 体诱导剂-2 (Autoinducer-2, AT-2)情况进行了检测，7株乳酸菌中检测到明 显的AI-2信号分子，且其屮3株产生AI-2信号极强;对高产AI-2的菌株，通过 RT-PCR对乳酸菌luxS基因和LDH基因的表达情况进行了检测，发现高产AI-2 的乳酸菌其luxS基因表达较强，而LDH基因表达较弱；最后分析了酸性环境与乳 酸菌信号分子AI-2产量的相关性，结果表明培养基酸性越强，乳酸菌产AI-2 信号越强o luxS基因是A卜2信号合成的关键基因，人卜2信号分子提高了乳酸菌 的耐酸性，有利于乳酸菌通过群体感应信号调控肠道内的酸碱平衡和菌群平衡。 本研究为后续解析乳酸菌群体感应信号传导通路、探索乳酸菌在宿主肠道内粘附 定植的机制奠定基础。 关键词： 乳酸菌;群体感应;AI-2; luxS基因；RT-PCR; 作者简介：蔡针华，硕士研究生，主要从事生物化学与分子生物学研究。 E-mail:1135887646@qq. com 作者简介：贾震虎，副教授，博士，主要从事食品微生物等方面的研究。 E-mail:jiaxueshi@163. com 收稿日期：2017-09-01 基金：国家自然科学基金面上项冃 Screening and characterization research of quorum sensing signaling molecule AI-2 high-yield Lactobacillus Strains CAI Zhen-hua CHENG Na JIA Zhen-hu PANG Xiao-yang School of Life Science, Shanxi Normal University; Institute of food science and technology CAAS; Abstract： Lactobacillus quorum sensing system is an important regulatory pathway for lac tic acid bacteria to respond to environmental changes and regulate the expression of related genes in vivo. Tn order to study the quorum sensi ng system of lac tic acid bacteria, In t his st udy, AI~2 was detected in 8 st rains of lactic acid bac tc ria, and 7 st rains of lac tic acid bac tc ria were detected in the AI-2 signal molecules, and 3 of them produce AI-2 signal is very strong. For strains with high yield AI-2, the expression of luxS gene and LDH gene of lactic acid bacteria were detected by RT-PCR. The expression of luxS gene was higher in lac tic acid bacteria with high yield of AI-2, and the expression of LDH gene was weaker. Finally, the correlation between acid environment and Al-2 production of lactic acid bacteria was analyzed. The results showed that the stronger the acidity of the culture medium, the stronger the AI-2 signal.LuxS gene is the key gene of AI-2 signal synthesis. Al-2 signal molecule improves the acid resistance of lac tic acid bac te