补充烟酸及耐力训练对急性酒精性肝损伤大鼠SirT1活性及表达影响及机制探究.docxVIP

补充烟酸及耐力训练对急性酒精性肝损伤大鼠SirTl活性及表达影响及机制探究 摘 要：目的：研究补充烟酸及耐力训练对急性酒精性肝 损伤大鼠SirTl表达、活性的影响及机制。研究方法：以 Wistar大鼠建立急性酒精性肝损伤模型，以跑台运动为训练 手段，并且给予大鼠补充烟酸，测定血清ALT和AS以及肝 脏MnSOD活性、NAD +/NADH比值、MDA含量、SirTl活性、 线粒体ATP合成活力、MnSOD和SirTlmRNA表达。结果：急 性酒精摄入导致血清ALT和AST水平、肝脏MDA含量均显著 升高，线粒体ATP合成活力、肝脏NAD +/NADH比值、MnSOD 及SirTl活性及mRNA表达均显著降低；耐力训练及补充烟 酸再给予酒精摄入与只给予酒精摄入血清ALT和AST水平、 肝脏MDA含量、线粒体ATP合成活力、肝脏NAD +/NADH比 值、MnSOD及SirTl活性及mRNA表达变化规律一致，但变化 幅度相对较小。结论：耐力训练及补充烟酸均能提高肝脏细 胞内NAD +/NADH的比值，使SirTl活性及表达升高，进而 提高肝脏细胞抗氧化能力，达到预防/改善急性酒精性肝损 伤的目的。 关键词：烟酸；耐力训练；急性酒精性肝损伤；沉默信 息调节因子 中图分类号：G804. 7文献标识码：A文章编号: 1006-2076 (2012) 06-0068-05 Abstract： Objective： To investigate the influence training on theof niacin supplement and enduranee activity and expression of SirTl and its mechanism in alcohol-induced acute hepatic injury in rats. Methods： training on the in serum ,It analyzed the level of ALT and AST in serum , NAD +/NADH , content of MDA , mitochondrial ATP synthesis, activities and expressions of MnSOD and SirTl in liver tissue in alcohol-induced acute hepatic injury rats after treadmill training and niacin supplement? Results : The acute alcohol treatment increases ALT and AST in serum and content of MDA in liver tissue, decreases mitochondrial ATP synthesis, ratio of NAD +/NADH, activities and expression of MnSOD and SirTl in alcohol-induced acute hepatic injury rats. The parameters of the above indicates the same after endurance training and niacin supplement but the extent is relatively lower .Conclusion : Endurance training and niacin supplement can increase ration of NAD +/NADH , which improves the activity and expression of SirTl, and then increases antioxidant ability of liver, which achieves the goal of protecting or improving alcohol—induced acute hepatic injury. Key words : niacin ； endurance training ； alcohol-induced acute hepatic injury ； silent information regulator 收稿日期：2012-07-20 作者简介：宋信勇(1965-),男，硕士，副教授，研 究方向体育教育训练学。 作者单位：山东体育学院，山东济南250102 Shandong Sport University, Jinan 250102, Shandong, China