生物医学工程技术Chapter 4 Electron Microscope and Flourescence probes.pptx

Chapter 4 Electron Microscope and Flourescence probes;Electron Microscope (EM);1. Basic of Electron Microscope;;2. Electrostatic Condenser;;3. Electron/Sample Interactions;;Transmission Electron Microscope (TEM);The construct of TEM;Ruska的博士论文;;Copper grid;;Scanning Electron Microscope (SEM);;Scanning Electron Microscope (SEM);Secondary electron, 50 eV;structure of SEM: Electron gun; Electron optical system; Detecting system; Imaging system. Deflector coils (偏转线圈) bend the primary beam for scanning across the specimen. Deflector coil in SEM connect to the CRT deflector plates;SEM accelerating voltages are limited to around 40 KV;Remove all water, solvents, or other materials that could vaporize while in the vacuum. Firmly mount all the samples. Non-metallic samples, such as bugs, plants, fingernails, and ceramics, should be coated so they are electrically conductive. Metallic samples can be placed directly into the SEM.;;;;;;Advantages of SEM;Inorganic Fluorescence Probes;Quantum dots (量子点);量子点限域效应研究基于早期水相和简单的有机相合 成的实验结果;Quantum dots (量子点);Brus L. E. Alivisatos A. P., Peng X. G.; Bawendi M. G.; Nie S. M. Weller H.;;量子点吸收谱宽、发射谱对称，可同时激发;Photo-bleaching p149;水相合成;;;First bioapplication of QDs, cell imaging;;TEM and Fluorescence images Of Cell;lymphatic imaging 淋巴;Our recent research results;;DNA detect p154-156;;A. Paul Alivisatos et al., nature, 2005, 430, 119,;Other Fluorescence inorganic nanomaterials;;;;量子点荧光探针小结