药物靶向调控ID4基因表达的生物信息学预测与分析.docVIP

药物靶向调控ID4基因表达的生物信息学预测与分析 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：药物靶向调控ID4基因表达的生物信息学预测与分析 2 文2：基因结构及其表达的调控 7 第一部分　 知识拓展 7 一、基因的结构 7 （一）原核细胞的基因结构 7 （二）真核基因是不连续基因 8 1、实例：鸡卵清蛋白mRNA与DNA杂交实验 8 2、真核基因的结构 8 （1）基因由编码区和非编码区两部分组成 8 二、基因表达的调控 8 （一） 原核基因表达的调控 8 (二)真核基因表达的调控 9 1、DNA含量高和基因数目多，且与其他物质组成核小体 9 3、前体RNA需要剪接才能成为有功能的成熟的信使RNA。 9 (三)人类基因组计划 9 1、人类只有一个基因组 9 (3)携带着人类的全部遗传信息 10 2、人类基因组计划绘制4张图 10 (4)序列图：测定人类基因组的DNA序列是核心 10 3、人类基因组计划的意义 10 第二部分　 例题讲解 11 （4）你认为完成“人类基因组计划”有什么意义？ 13 第三部分　 单元练习 13 1、下列对基因结构的认识中，不正确的是： 13 3、基因是由 14 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 17 正文 药物靶向调控ID4基因表达的生物信息学预测与分析 文1：药物靶向调控ID4基因表达的生物信息学预测与分析 Bioinformatics Scan Analysis for Predicting Drug Targeted Modulation on ID4 Gene Expression Abstract Low expression of ID4 gene is tightly related with carcinogenesis and high expression shows a definite antileukemia effect, though little expression in some leukemia cells. The main purpose of this preliminary work was to analyze the cotruction of ID4 gene promoter and to predict the cis elements in the ID4 promoter region by scanning the drug candidate with bioinformatics method. All these work are the primary part for finding effective drugs in the treatment of leukemia via the way of ID4 expression regulation. According to the data in GenBank and Internet platform, the 5untralated sequence just upstream of ID4 ORF was virtually cloned. TESS, Genomatix and GenBank databank were used to analyze the cis elements in this area. A was used to find the distribution patter for all these possible elements. SAGE and GEO datasets were used to find active substances which have the effect on the ID4 expression. The ults indicated that ID4 had a type II promoter with a typical TATA box45 bp upstream the tracriptional original site. There were a lot of various cis elements in the 5untralated region upstream, including both positive element candidates such as Sp1, cMyb, abaA, GR, ER, Zeste