稳定沉默TIPE2后削弱PolyIC诱导的人单核巨噬细胞凋亡.docVIP

稳定沉默TIPE2后削弱PolyIC诱导的人单核巨噬细胞凋亡 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：稳定沉默TIPE2后削弱PolyIC诱导的人单核巨噬细胞凋亡 1 2 结果 6 3 讨论 8 文2：PolyIC诱导C57BL6小鼠产生自身免疫样病变 10 1 材料和方法 10 2 结果 11 3 讨论 12 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 稳定沉默TIPE2后削弱PolyIC诱导的人单核巨噬细胞凋亡 文1：稳定沉默TIPE2后削弱PolyIC诱导的人单核巨噬细胞凋亡 [Abstract] Objective To investigate the influence of stably silent TIPE2 on THP-1 apoptosis induced by TLR agonist Poly I：C. Methods THP-1 cells were selected as the study subjects. The expression of TIPE2 mRNA was detected by fluorogenic quantitative PCR after incubation of THP-1 cells with different TLR activating agents. After stable silence of TIPE2 by lentiviral technique， the cell proliferation was detected by MTT， apoptosis was detected by Annexin V/PI double-staining method， and changes of Caspase-8 expression level after silence of TIPE2 was analyzed by western blot. Results TLR3 specific activator Poly I：C could significantly up-regulate the expression of TIPE2（P）. Along with the time of action， it could significantly inhibit the proliferation of THP-1， and induce apoptosis. Stable silence of TIPE2 in THP-1 by lentiviral technique could significantly impair the TIPE2 expression induced by Poly I：C （P）. After silence of TIPE2， the effects of Poly I：C on inhibition the proliferation of THP-1 and induction of apoptosis were significantly impaired， and the result of following western blot showed active form of Caspase-8 and down-regulation of p41/42 expression. Conclusion By up-regulating the expression of TIPE2， Poly I：C can inhibit the proliferation of THP-1， and induce apoptosis， which can be partially impaired by stable silence of TIPE2， possibly due to down-regulation of Caspase-8 active form expression。 [Key words] Poly I：C； TIPE2； Immune； TLR； Apoptosis 免疫系统内稳态的维护机制极其复杂和神秘，改变调控免疫细胞死亡或者活化扩增免疫细胞均可以打破免疫细胞的内稳态环境，最终带来极为致命的炎性疾病，目前研究发现一组超家族蛋白对免疫系统的内稳态维持至关重要。尤其是具有hexahelical bundle 结构的蛋白称之为死亡效应domain（DED），与Caspase家族蛋白的CARD结构域以及募集区域有结构类似，参与到细胞死亡和其他的信号通路中。