IFN- on MMP-2 -9 and TIMP-1 in HL-60 cells expression of.docVIP

 PAGE \* MERGEFORMAT 17 IFN- on MMP-2 -9 and TIMP-1 in HL-60 cells expression of Study: Heart Liu, Ting Wang, Yang Hua, Chen Wei, Xu Bo [Abstract] Objective To study the leukemia cell line HL-60 matrix metalloproteinase 2,9 (MMP-2, MMP-9) and the natural inhibitor of matrix metalloproteinase -1 (TIMP-1) expression and cytokine interferon (IFN -) on MMP-2, MMP-9 and TIMP-1 transcription regulation. Methods HL-60 cell growth in different periods (1-5d) cells were collected, using semi-quantitative RT-PCR method, analysis of leukemia cells line HL-60 in the MMP-2, MMP-9 and TIMP-1 mRNA expression levels, with different concentrations of IFN- effect on HL-60 cells, 24,48 h after the RT-PCR, MMP-2, MMP- 9 and TIMP-1 mRNA levels. Results after cell inoculation 24 h MMP-2 and MMP-9 in the mRNA level of the highest, but the relatively constant expression of TIMP-1. IFN- in various concentrations of MMP-2 and MMP-9 mRNA expression was inhibited (P lt;0.05), and with the effect of increasing the concentration of reduced mRNA expression, TIMP-1 is not sensitive to its regulation. Conclusions clear leukemia HL-60 cell line expression at the transcriptional level MMP-2, MMP -9 and TIMP-1, IFN- on MMP-2, MMP-9 mRNA expression was inhibited, while transcription of TIMP-1 had no significant effect. [Keywords:] matrix metalloproteinase, matrix metalloproteinase inhibitors, leukemia, interferon- ABSTRACT: Objective To detect the expression of MMP-2, MMP-9 and TIMP-1 in leukemia cell line HL-60, and explore the effect of interferon- (IFN-) on their expression. Methods Reverse transcriptase-polymerase chain reaction was used to assay MMP-2, 9 and TIMP-1 mRNA in leukemia cell line HL-60 at different time (the first day to the fifth day of the cell growth), and half-quantity values of the influence on the cells after treatment with IFN- in different concentration and different time (24 hours, 48 hours). Results The expression of MMP-2, -9 mRNA was significantly the hig