脂多糖对人腹膜间皮细胞葡萄糖转运蛋白1表达的影响.pdfVIP

mesothelial which is cens(HPMCs)，in glucose glucoseffansporter一1primary inHPMCs．Ithasnotbeen thathowthe of transporter reportered expression GLUT／changeduringperitonitis．Bystudiiag the of to HPMCsand HPMCs try lating observingexpressiong GLUTl，thisstudy revealthe ofUF． mechanism Methods for of wasused cultureand 1．Enzymatic primary disaggregation passage HPMCs． for 2．IndirectiinmunofluorescencewasusedidentificationofHPMCs． 3．GLUTl wasdetected theresult expression byimmunocytochemistry，and was analyzedbyimageanalyticalsystem． 4．GLUTlmRNAwasdetectedreverse chain by transcriptionpolymerase reaction(RT—PCR)technology． 5．Hexokinasewasusedformeasurementofconcentrationofglucose assay incellculture thennet utilizationwascalculated． fluid，and glucose 6．Statistical datawere withSPSS10．0．Ttestand analysis：Allprocessed between alldatawere ANOVAwasusedtotestthedifferencesgoups，and pres— entedas曼±s．P0．05wasconsideredtobe statisticallysignificant． Reslllts of immunofluores- 1．Identificationof resultindirect