胞核内高表达M―CSF对HeLa细胞生长增殖影响.docVIP

胞核内高表达M―CSF对HeLa细胞生长增殖影响 　　[摘要] 目的 分析人宫颈癌HeLa细胞核内M-CSF增高对化疗药物的影响。 方法 体外培养HeLa细胞、HeLa-M细胞以及转染M-CSF空载体HeLa-C细胞。分别使用紫杉醇和顺铂（浓度分别为0.3、3、30 μg/mL）作用48 h，采用MTT方法比较细胞的相对活性，Westen blot分析转染M-CSF对抗凋亡分子Bcl-2表达的影响。 结果 当两种化疗药物的浓度为3、30 μg/mL时，与HeLa-C细胞和HeLa细胞相比，HeLa-M细胞活性明显增高，差异有统计学意义（P0.05）；当浓度为0.3 μg/mL时三种细胞活性无明显差异。Westen blot结果显示，转染M-CSF后能够使细胞中的抗凋亡蛋白Bcl-2表达水平明显升高。 结论 人宫颈癌HeLa细胞核中的M-CSF增高可以使细胞的药抗性提高，从而抑制HeLa细胞的凋亡。 　　[关键词] 人宫颈癌；HeLa细胞核；M-CSF；化疗药物 　　[中图分类号] R73 [文献标识码] A [文章编号] 1673-9701（2017）16-0030-03 　　[Abstract] Objective To analyze the effect of elevated M-CSF in nuclei on the chemotherapeutic drugs of human cervical cancer HeLa cells. Methods HeLa cells， HeLa-M cells and HeLa-C transfected with M-CSF empty vector were cultured in vitro. The paclitaxel and cisplatin（concentrations were 0.3， 3， 30 μg/mL） were used for 48 h， respectively. The relative viability of the cells was compared by MTT method. The effect of transfection with M-CSF on the expression of Bcl-2 was detected by Western blot analysis. The effect of paclitaxel and cisplatin （concentration 0.3， 3， 30 μg/mL） on the expression of anti-apoptotic molecule Bcl-2 was detected by MTT method. Results The viability of HeLa-M cells was significantly higher than that of HeLa-C cells and HeLa cells when the concentration of the two chemotherapeutic drugs was 3， 30 μg/mL， and the difference was statistically significant（P0.05）. There was no significant difference in cell viability between the three kinds of cells when the concentration was 0.3 μg/mL. The results of Western blot showed that the expression of anti-apoptotic protein Bcl-2 was significantly increased after transfection with M-CSF. Conclusion The increase of M-CSF in the nucleus of human cervical cancer HeLa cells can improve the drug resistance of the cells and inhibit the apoptosis of HeLa cells. 　　[Key words] Human cervical cancer； HeLa nucleus； M-CSF； Chemotherapy drugs 　　M-CSF即巨噬?胞集落刺激因子，简称集落刺激因子[1]，主要是由链间二硫键所形成的同源二聚体糖蛋白，其与CSF-1R相互作用会使受体发生二聚化反应，随后可以与