黄精提取液对老龄小鼠心脑SOD和MDA含量影响.docVIP

黄精提取液对老龄小鼠心脑SOD和MDA含量影响 　　[摘要] 目的:探讨黄精提取液对老年小鼠的抗衰老作用。方法:将老龄小鼠随机分为黄精提取液高剂量组(15 g/kg)、中剂量组(10 g/kg)、低剂量组(5 g/kg)和老龄生理盐水(5 g/kg)对照组,每组6只,四组连续灌胃21 d,1次/d,第22天以颈椎脱臼法处死小鼠,迅速取其心、脑组织并测定其超氧化物歧化酶(SOD)及丙二醛(MDA)含量。结果:除高剂量组脑组织SOD含量升高不明显外,其余各黄精提取液实验组心、脑SOD含量均较对照组明显升高(P0.01),其中以低量组变化最明显。黄精提取液实验组心、脑组织中MDA含量与对照组相比均有所降低,其中中剂量组差异有高度统计学意义(P0.01),高剂量组差异有统计学意义(P0.05),低剂量组变化不明显。结论:黄精提取液具有抵抗和清除老龄小鼠机体自由基的作用,可延缓衰老进程。 　　[关键词] 黄精提取液;超氧化物歧化酶(SOD);丙二醛(MDA);老龄小鼠 　　[中图分类号] R285.5 [文献标识码]B[文章编号]1673-7210(2010)03(c)-031-02 　　 　　Effect of Polygonatum Extraction on heart and brain SOD and MDA content in mice aging 　　WEI Suzhen1, HUANG Yanlian1, LU Yixun1, HUANG Guixiong1, ZHAO Shanmin2△, JIN Ling2, HUANG Yanfeng2 　　(Youjiang Medical College for Nationalities, Baise 533000, China) 　　[Abstract] Objective: To investigate the Polygonatum extract of the older anti-aging effects in mice. Methods: The aging mice were randomly divided into Polygonatum Extraction high-dose group(15 g/kg), middle-dose group (10 g/kg), low-dose group (5 g/kg) and aging normal saline (5 g/kg) control group, with six mice in each group, four consecutive fed 21 d, 1 time per day, in the 22ed mice were killed by cervical dislocation method to quickly draw upon the heart, brain and measured superoxide dismutase (SOD) and malondialdehyde (MDA) content. Results: In addition to high-dose group increased in brain tissue SOD content was not obvious, the rest of the Polygonatum Extraction from the heart of experimental group, brain SOD concentration was significantly higher than those of control group (P0.01), of which the most obvious changes in low-dose group. Compared with the control group, both MDA levels in heart and brain tissue of Polygonatum Extraction group were decreased, the middle-dose group and the high-dose group were significantly lower (P0.01 or P0.05), low-dose group did not change significantly. Conclusion: Polygonatum Extraction plays a role in the resistance and removal of free radicals in old mice, and can de