黄精提取物对耐力训练大鼠骨骼肌组织NONOS体系影响实验研究.docVIP

黄精提取物对耐力训练大鼠骨骼肌组织NONOS体系影响实验研究 　　摘要：目的: 探讨黄精对不同负荷耐力训练大鼠骨骼肌组织中一氧化氮(NO)及一氧化氮合酶(NOS)体系的影响，为黄精作为运动补剂的开发提供实验依据。方法：选取雄性大鼠24只，随机分为（A组）、运动对照组（B组）、运动+黄精提取物组（C组），B组和C组按照训练模型进行为期6周的不同负荷的跑台训练，并测定大鼠骨骼肌组织中一氧化氮（NO），总一氧化氮（T-NOS），结构性型一氧化氮(cNOS)和诱导型一氧化氮(iNOS)活性以及力竭时间。结果：黄精提取物可以促进骨骼肌组织中产生生理剂量的NO；升高T-NOS、cNOS活性；抑制大强度力竭性运动导致的iNOS过量表达；延长大鼠运动至力竭的时间。结论：黄精提取物可以影响不同运动负荷状态下骨骼肌组织NO-NOS的变化，改善组织代谢状况，提高运动能力。 　　关键词：黄精；耐力训练；一氧化氮；一氧化氮合酶 　　中图分类号：G804.7文献标识码：A文章编号：1007-3612(2008)09-1225-03 　　 　　Experimental Research on the Effect of Polygonatum sibiricum Red.to NO-NOS System in 　　Skeletal muscles Tissues of Endurance-trained Rats 　　MAO Yan?1, HAN Xiao-yan?1, XIONG Zheng-ying?2, LIU Xiao-jun?3 　　(1.Department of physical education, Xi.an University of Architecture Technology, Xi.an 710055, Shaanxi;2.Institute of 　　Sport Biological Research, Shaanxi Normal University, Xi.an 710061, Shaanxi China; 　　3.Department of Physical Education, Weinan Normal College, Weinan 714000, Shaanxi China) 　　Abstract:Purpose: This study discussed the mechanism of Polygonatum sibiricum Red impacting to myocardial mitochondrial antioxident ability and system of NO-NOS in trained- indurance Rats, which can provide examination base for using Polygonatum sibiricum Red as sport supplements. Methods: Twenty-four old male SD rat were randomly divided into four groups. Group B and C rats were experimented by building the Endurance-trained exercise model in mice to probe into the activities of the nitrous oxides (NO) and synthase (NOS) of skeleton muscle through 6-week different load running in the platform. Results: It indicate that the Polygonatum sibiricum Red can increase the activities of NO, T-NOS, cNOS activity; Inhibit the over-expression by intensity strength;Lengthen the time that the great mouses movement exhausts to strength. Conclusion: Polygonatum sibiricum Red could influence the NO-NOS systems change of skeleton muscle under the different load, improve movement ability. 　　Key words: Polygonatum sibiricum