葛根素及大豆苷元的分离纯化工艺及大豆苷元固体分散体片的研制-药物化学专业论文.docxVIP

贵州大学硕士研究生学位论文 贵州大学硕士研究生学位论文 PAGE PAGE 4 图书分类号： ABSTRACT This experiment makes systemic research on joint production process of Daidzein and Puerarin,then makes a systematic study on Daidzein of solid dispersion . Main content summed up as follows: Hydrolysis puerarin isoflavone. The acid hydrolysis method can be the puerarin consociation sibeline and yufengningxin soybean isoflavone puerarin hydrolysis into derivatives yuan RMB, soybean and through orthogonal experiment of hydrochloric acid hydrolysis puerarin isoflavones have optimum reaction conditions for: hydrochloric acid concentration2.5mol·L-1, hydrolysis time 90min, hydrolysis, hydrochloric acid 90 degrees Celsius temperature than 14 for volume and raw materials. This experiment using optimal hydrolysis reaction conditions, can make the puerarin consociation sibeline rises up to 25% , Daidzin than 99% conversion. Separation and purification of puerarin and Daidzein. Acid hydrolysis places the isoflavone, cooling, with sodium hydroxide adjust pH 1.5. Ethyl acetate extract Daidzein, remaining enrichment soybean water part, precipitation, precipitating Puerarin. Use 50% ethanol crystallization 2-3 times, can get yellow powder material Daidzein, With 90% acetic crystallization 2-3 times, can get colorless needle crystal Puerarin. The methods of extraction and separation from pueraria and Daidzein has simple operation, high yield and purity, and low cost. Puerarin and Daidzein respectively,2.5% ,14.8% ; purity 97.3% and 97.1%. To determine the equilibrium solubility of daidzein in various medium and its partition coefficients for the n-Octanol-water/bufer solution systems. A high performance liquid chromatography method was established and used to detect the concentration of daidzein in water and bufer solutions; the partition coefficients for the n-Octanol-water/bufer solution systems of daidzein were determined by shaking flask method. The equilibrium solubility of daidzein in water was 0.78mg·L-1