雷公藤多甙对急性移植物抗宿主病小鼠的作用.docVIP

雷公藤多甙对急性移植物抗宿主病小鼠的作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：雷公藤多甙对急性移植物抗宿主病小鼠的作用 1 文2：昆明山海棠提取物对移植物抗宿主病小鼠T细胞亚群的影响 10 1 材料和方法 11 2 结果 12 3 讨论 14 参考文摘引言： 15 原创性声明（模板） 17 文章致谢（模板） 18 正文 雷公藤多甙对急性移植物抗宿主病小鼠的作用 文1：雷公藤多甙对急性移植物抗宿主病小鼠的作用 Glucosidorum Tripterygii Tororum (GTT) is a kind of Chinese traditional medicine (CTM), for the fit time used in China as an immunoregulatory agent which is often used in the treatment of autoimmunological diseases such as rheumatoid arthritis (RA) and nephrotic syndrome. Our previous experiment showed that GTT can decrease the population of lymphocytes, inhibit the secretion of IL-2 and expression of IL-2R, down-regulate the seitivity of T cells to IL-2, and inhibit the expression of adhesion molecules (CD11a, CD18) on the surface of T cells. And it can prolong the survival time of allogeneic traplanted skin. Its effect on anti-rejection is weaker than CsA and stronger than methylprednisolone. And, the combination of GTT with them shows a much more stronger effect, indicating that GTT can moderately or obviously inhibit the function of T cells and the secretion of cytokines［1］. Furthermore, GTT may have anti-inflammatory and anti-tumor effects. Graft-Veus-Host disease (GVHD), especially acute Graft-Veus-Host disease (aGVHD) is a major lethal complication after bone marrow traplantation (BMT). Therefore, this study was aimed to adopt GTT or combine GTT with CsA in order to explore the role of GTT on aGVHD and its mechanism. Materials and Methods Mice Donor mice BALB/c(H-2d), female; syngeneic traplantation recipient mice BALB/c(H-2d), male; allogeneic traplantation recipient mice C57BL/6(H-2b) male. All of them were 8-12 weeks old and weight of about 20 g (provided by Ititute of Zoology, Chinese Academy of Sciences) Induction of aGVHD The recipients received whole body irradiation by a li- near accelerator (total dose is 1800 cGy, rate of dose is 200 Gy/min, SIMENZ, Primus, Germany) for 6-8 hou before traplantation. B