TPA诱导K562细胞分化过程中线粒体铁蛋白表达情况研究.docVIP

TPA诱导K562细胞分化过程中线粒体铁蛋白表达情况研究 正文 TPA诱导K562细胞分化过程中线粒体铁蛋白表达情况研究 文1：TPA诱导K562细胞分化过程中线粒体铁蛋白表达情况研究 Expression of Mitochondrial Ferritin in K562 Leukemic Cell du ring TPAInduced Cell Differentiation Abstract Mitochondrial ferritin (MtF), a new player in iron metabolism, fit identified in 2001, is highly homologous to ferritin both structurally and functionally. Preliminary studies have suggested that MtF might play very important roles in the regulation of mitochondrial iron homeostasis. Leukemic cells, just like other malignant cells, demand more iron for their greater proliferation potential. However, little is known about what roles MtF might play in leukemic cell iron metabolism and cell proliferation. The aim of this study was to investigate the expression of MtF, traferrin receptor 1 (TfR1) and ferritin (Fn) mRNAs in K562 leukemic cells during TPAinduced cell differentiation and to explore the interrelatiohip between the expression levels of these iron metabolismrelated molecules. K562 cells cultured with or without TPA (16 nmol/L) were collected at 24, 72 and 120 hou respectively. Cell differentiation toward monocyte lineage was confirmed by microscopic study (Wrights staining) and flow cytometry. Semiquantitative RTPCR was performed to determine mRNA expression, with housekeeping gene βactin as control reference. This study revealed that over 95% of K562 cells showed morphological features of monocyte/macrophage，and the expression of CD64 on cell surface increased significantly at day 5 with TPA treatment. K562 cells could express a certain level of MtF before TPAinduced differentiation. With increase of TPAinduced cell differentiation, MtF mRNA expressio were downregulated progressively. After 5 days of induced cell differentiation， expression levels of MtF and TfR1 mRNA were just % and % of that before TPA treatment. While Fn mRNA expression increased to folds of that before TPA treatment. It is concluded that MtF expression is downregulated during TPA