HPLC Determination of Astragalus leaves the content of Astragaloside.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Determination of Astragalus leaves the content of Astragaloside [Abstract] Objective To establish an accurate, simple and determination of astragaloside content analysis method. Method column of Nucleosil C18 column (4.6mm * 250mm, 5μ m); mobile phase of acetonitrile - water = 1:2; a flow rate of 0.8ml/min; detection wavelength 205 nm; speed 0.4mm/min. Sensitivity of 0.1 AUFS, was determined by the content of astragaloside average recovery was 96.1%, RSD of 2.15%. The results of the sample concentration of 0.01 ~ 0.2mg/ml and the peak area between the good linear relationship. Conclusion This method of repeatable, high precision, strong stability. [Keywords:] HPLC Astragalus Astragalus leaves leaves Astragaloside [Abstract] 0bjective: Establishes one kind accurately, easily to determine Astragaloside the content the analysis method. Method: The chromatographic column is Nucleosil the C18 column (4.6mm * 250mm, 5μ m); Flowing for methyl cyanide - water = 1:2; The speed of flow is 0.8ml/min; The examination wave length is 205 nm; The speed is 0.4mm/min. The sensitivity is 0.1 AUFS, has determined the Astragaloside content. The average returns-ratio is 96.1%, RSD is 2.15 %. Result: Sample density in 0.01 ~ 0.2mg/ml becomes the good linear relationship with the peak area.Conclusion: This method’s duplication is good, accuracy high, stable. [Keywords:] HPLC Leaf of the Astragalus Astragaloside Astragalus is commonly used traditional Chinese medicine, known as ‘tonic long’ is known, with Qi-solid form, the effectiveness of diuretic drugs, and other care. Astragalus high medicinal value, used clinically for the treatment of non-specific immune dysfunction, hepatitis B and the cardiovascular system disease. Astragaloside one of the components as the astragalus, which has blood pressure, anti-inflammatory, stable red cell membrane, increase plasma CAMP levels, promoting DNA synthesis in regenerating liver in mice and enhanced immune func