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酶解法提取南极磷虾虾油的研究 Extraction of Antarctic krill oil by enzymatic hydrolysis
2013年第38卷第3期 中国油脂 l
酶解法提取南极磷虾虾油的研究
周长平1，孙军涛1，王洪新1’2，马朝阳1，王远辉1，娄在祥1
(1．江南大学食品学院，江苏无锡214122；
2．江南大学食品科学与技术国家重点实验室，江苏无锡214122)
摘要：采用酶解法结合溶剂萃取从南极磷虾中提取虾油，通过对比实验，选择了先加碱性蛋白酶再
加中性蛋白酶的加酶方法，重点研究了两种酶的添加量和酶解时间对提取率的影响。经单因素实
8、55℃
验及正交实验确定的最佳工艺条件为：固定料液比1：3．4，先加入0．1％碱性蛋白酶在pH
下酶解2 7、50℃下酶解2．5
h，再加入O．1％中性蛋白酶在pH h。该条件下虾油的提取率为
64．52％。提取的虾油酸值(KOH)为
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