进出口食品中荷叶碱的测定英文意见稿.docVIP

Foreword This standard was drafted according with GB/T 1.1-2009 Directives for standardization-Part1：Structure and drafting of standards. Please note that some of the content of this document may involve patents, the publisher of this document does not assume the responsibility to identify these patents. This standard was proposed by and is under the charge of the Certification and Accreditation Administration of the People?s Republic of China. This standard was drafted by the Hunan Entry-Exit Inspection and Quarantine Bureau of the People?s Republic of China and Xiamen Entry-Exit Inspection and Quarantine Bureau of the People?s Republic of China. The standard was mainly drafted by This standard is a professional standard promulgated for the first time. Note: This English version, a translation from the Chinese text, is solely for guidance. Determination of nuciferine in health foods for import and export—— HPLC-MS/MS method Scope This standard specifies the sample preparation and determination of nuciferine in health foods for import and export by HPLC-MS/MS method. This standard is applicable to the determination and the confirmation of nuciferine lipid-lowering capsule, diet chewable tablet and diet tea. Cited normative documents The following normative documents are indispensable to the application of this standard. For dated references, only the edition bearing such date applies to this standard. For undated references, the latest edition of the normative document referred to (including all the amendments) applies. GB/T 6682 Water for analytical laboratory use specification and test methods. Abstract of method Nuciferine in sample is extracted with methanol in a ultrasonic washer. Then the extract is diluted and filtered off is filtered through the 0.45 μm membrane filter. The filtrate is determined by HPLC-MS/MS. External standards method is used for quantitative measurement. Reagents and materials Unless otherwise specified, all the reagents should be of