HPLC determination DAXXK capsules diosgenin content_0.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination DAXXK capsules diosgenin content [Abstract] Objective: To establish DAXXK capsules accurate quantitative quality control methods. Methods: The high-performance liquid chromatography (HPLC), with 18 alkyl silane bonded phase silica gel as stationary phase; HPLC pure methanol as the mobile phase; detection wavelength 210nm; the flow rate of 0.6ml.min, measured DAXXK capsule main elements content of diosgenin. Results: DAXXK capsules diosgenin sample volume of 0.6375 ~ 10.2000μ g range with the peak area showed good linear relationship between the average recovery was 98.2%, RSD 1.7%. Conclusion: The method is simple, convenient and good reproducibility is used to control DAXXK high levels of credibility. [Keywords:] DAXXK; diosgenin; Determination DAXXK capsule with blood circulation of qi and pain relief, expansion of coronary blood vessels, improving the role of myocardial ischemia, etc.; for the prevention and treatment of coronary heart disease, angina, and bleeding resistance of the Chest, dizziness, shortness of breath, palpitations, chest tightness or pain [1], the clinical application of a wide range; the current Pharmacopoeia method for the determination hydrolysis method, accuracy is not high, poor reproducibility, the application of practical work over there is the inconvenience, because DAXXK Capsule major components diosgenin [2], this switch to high-performance liquid chromatography, on the Determination of diosgenin had methodological study tests; The method is simple, convenient and good reproducibility is used to control DAXXK capsules credibility of high, results were satisfactory. An instrument and reagent 1.1 Instrument Shimadzu LC10ATvp high-performance liquid chromatography (UV-Vis detector); N2000 chromatography workstation; Kromasil C18 reversed-phase column (4.6mm * 250mm, 5μ m); Sartorius BP211-D electronic balance; EasypureRF Millipore . 1.2 Reagent water (using ultra-pure water prepara