HPLC Determination of Gatifloxacin in the content of polylactic acid nanoparticles.docVIP

 PAGE \* MERGEFORMAT 10 HPLC Determination of Gatifloxacin in the content of polylactic acid nanoparticles Authors: DENG, LIANG Jianjian, Zhang Shu, Lu Shang-shift [Abstract] Objective To establish the gatifloxacin polylactide nanoparticles by HPLC method. Methods RP-HPLC column was ODS  C18 column (150 mm * 4.6 mm, 5 μ m), mobile phase was 0.02 mol / L citric acid solution (containing 0.6% triethylamine)  methanol (volume ratio 60:40), detection wavelength 290 nm, flow rate 1.0 mL / min, injection volume 20 μ L. The results of gatifloxacin in the 4.010 ~ 60.15 μ g / mL concentration and peak area within the linear relationship was good (r = 1.000), the average recovery was 100.2%, RSD of 0.88% (n = 9). Conclusion The method can be used gatifloxacin determination of polylactic acid nanoparticles. [Keywords:] Polylactic acid nanoparticles; Gatifloxacin; high-performance liquid chromatography; determination Abstract: Objective To establish a RP  HPLC method for the determination of gatifloxacin in gatifloxacin  polylactic acid nanoparticles.Methods The analysis was performed on an ODS  C18 column (150 mm * 4.6 mm, 5 μ m) using 0.02 mol / L citric acid (0.6% triethylamine)  methanol (60:40) as mobile phase under the flow rate of 1.0 mL / min. The detection wavelength was 290 nm.Results Gatifloxacin had a good linear range of 0.0802 ~ 1.2030 μ g (r = 1.000). The average recovery rate was 100.2% with RSD 0.88% (n = 9). Conclusion The method can be used to detect the content of gatifloxacin in the nanoparticles. Keywords:: polylactic acid nanoparticles; gatifloxacin; HPLC Gatifloxacin (gatifloxacin) for the fourth-generation quinolone antibacterial drug, compared with existing quinolones, gatifloxacin antibacterial spectrum of large pairs of G bacteria, chlamydia, mycoplasma, mycobacteria, and anaerobes and others have a strong bactericidal effect, through the introduction of 8-methoxy reducing light toxicity, are safe and reliable next-generation f